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Analyst. 2018 Dec 3. doi: 10.1039/c8an01574k. [Epub ahead of print]

New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells.

Author information

1
Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA. thomas.metz@pnnl.gov.
2
Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA. thomas.metz@pnnl.gov and Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.

Abstract

Mass-spectrometry based omics technologies - namely proteomics, metabolomics and lipidomics - have enabled the molecular level systems biology investigation of organisms in unprecedented detail. There has been increasing interest for gaining a thorough, functional understanding of the biological consequences associated with cellular heterogeneity in a wide variety of research areas such as developmental biology, precision medicine, cancer research and microbiome science. Recent advances in mass spectrometry (MS) instrumentation and sample handling strategies are quickly making comprehensive omics analyses of single cells feasible, but key breakthroughs are still required to push through remaining bottlenecks. In this review, we discuss the challenges faced by single cell MS-based omics analyses and highlight recent technological advances that collectively can contribute to comprehensive and high throughput omics analyses in single cells. We provide a vision of the potential of integrating pioneering technologies such as Structures for Lossless Ion Manipulations (SLIM) for improved sensitivity and resolution, novel peptide identification tactics and standards free metabolomics approaches for future applications in single cell analysis.

PMID:
30507980
DOI:
10.1039/c8an01574k

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