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J Vis Exp. 2018 Nov 18;(141). doi: 10.3791/58499.

Subclavian Vein Puncture As an Alternative Method of Blood Sample Collection in Rats.

Author information

1
Department of Cardiology, The Second Xiangya Hospital, Central South University.
2
Department of General Surgery, The Second Xiangya Hospital, Central South University; zhougqin@21cn.com.
3
Department of Cardiology, The Second Xiangya Hospital, Central South University; zhoushenghua@csu.edu.cn.

Abstract

Blood collection with enough blood volume is essential in animal experiments. Blood collection from the tail vein of rats is popular and less stressful compared to other more aggressive methods such as retro-orbital plexus sample collection. However, this blood collection method is sometimes limited by an unsatisfactory success rate. Here, we introduce a method for blood collection through the subclavian vein puncture. The subclavian vein is located just under the clavicle and this vein is large enough to fulfill the volume requirements of blood collection. Our results show that this method is safe and applicable for blood collection sampling with the required blood volume. Blood collection through the subclavian vein puncture could serve as an alternative blood collection method in case of failed tail vein blood sampling in rats.

PMID:
30507916
DOI:
10.3791/58499
[Indexed for MEDLINE]

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