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J Clin Invest. 2019 Jan 2;129(1):310-323. doi: 10.1172/JCI95731. Epub 2018 Dec 3.

Chemogenetic silencing of hippocampal neurons suppresses epileptic neural circuits.

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Department of Neurosciences, Cleveland Clinic, Cleveland, Ohio, USA.
Department of Clinical Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, China.
Epilepsy Center, Neurological Institute, and.
Department of Quantitative Health Sciences, Cleveland Clinic, Cleveland, Ohio, USA.
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.


We investigated how pathological changes in newborn hippocampal dentate granule cells (DGCs) lead to epilepsy. Using a rabies virus-mediated retrograde tracing system and a designer receptors exclusively activated by designer drugs (DREADD) chemogenetic method, we demonstrated that newborn hippocampal DGCs are required for the formation of epileptic neural circuits and the induction of spontaneous recurrent seizures (SRS). A rabies virus-mediated mapping study revealed that aberrant circuit integration of hippocampal newborn DGCs formed excessive de novo excitatory connections as well as recurrent excitatory loops, allowing the hippocampus to produce, amplify, and propagate excessive recurrent excitatory signals. In epileptic mice, DREADD-mediated-specific suppression of hippocampal newborn DGCs dramatically reduced epileptic spikes and SRS in an inducible and reversible manner. Conversely, specific activation of hippocampal newborn DGCs increased both epileptic spikes and SRS. Our study reveals an essential role for hippocampal newborn DGCs in the formation and function of epileptic neural circuits, providing critical insights into DGCs as a potential therapeutic target for treating epilepsy.


Epilepsy; Neuronal stem cells; Neuroscience; Stem cells

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