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Front Microbiol. 2018 Nov 16;9:2689. doi: 10.3389/fmicb.2018.02689. eCollection 2018.

Comparative Transcriptomics Highlights New Features of the Iron Starvation Response in the Human Pathogen Candida glabrata.

Author information

1
Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, UMR 7238, Laboratoire de Biologie Computationnelle et Quantitative, Paris, France.
2
École Normale Supérieure, PSL Research University, CNRS, Inserm U1024, Institut de Biologie de l'École Normale Supérieure, Plateforme Génomique, Paris, France.
3
Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, UMR 7138, Évolution, Paris, France.
4
UMR 9198, Institute for Integrative Biology of the Cell, CEA, CNRS, Université Paris-Sud, UPSay, Gif-sur-Yvette, France.

Abstract

In this work, we used comparative transcriptomics to identify regulatory outliers (ROs) in the human pathogen Candida glabrata. ROs are genes that have very different expression patterns compared to their orthologs in other species. From comparative transcriptome analyses of the response of eight yeast species to toxic doses of selenite, a pleiotropic stress inducer, we identified 38 ROs in C. glabrata. Using transcriptome analyses of C. glabrata response to five different stresses, we pointed out five ROs which were more particularly responsive to iron starvation, a process which is very important for C. glabrata virulence. Global chromatin Immunoprecipitation and gene profiling analyses showed that four of these genes are actually new targets of the iron starvation responsive Aft2 transcription factor in C. glabrata. Two of them (HBS1 and DOM34b) are required for C. glabrata optimal growth in iron limited conditions. In S. cerevisiae, the orthologs of these two genes are involved in ribosome rescue by the NO GO decay (NGD) pathway. Hence, our results suggest a specific contribution of NGD co-factors to the C. glabrata adaptation to iron starvation.

KEYWORDS:

Aft; ChIP-seq; NO GO decay; evolution; yeast

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