Format

Send to

Choose Destination
Persoonia. 2018 Dec;40:135-153. doi: 10.3767/persoonia.2018.40.06. Epub 2018 Feb 19.

Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe.

Author information

1
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
2
Fera, Sand Hutton, York, YO41 1LZ, UK.
3
University of Idaho, Parma Research and Extension Center, Parma, Idaho, USA.
4
Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera s/n, 46022-Valencia, Spain.
5
Dipartimento di Scienze delle Produzioni Agroalimentari e dell'Ambiente (DiSPAA), Sezione di Patologia Vegetale ed Entomologia, Università di Firenze, Piazzale delle Cascine 28, 50144 Firenze, Italy.
6
Mendeleum - Department of Genetics, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44, Lednice, Czech Republic.
7
ARO The Volcani Center, 68 HaMacabim Road, Rishon LeZion, 7505101 Israel.
8
SFR Condorcet, Université de Reims Champagne-Ardenne, URVVC EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, Reims, Cedex 2 51687, France.
9
Instituto de Ciencias de la Vid y del Vino, Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, Gobierno de La Rioja, Logroño 26007, Spain.
10
Faculty of Natural and Environmental Sciences, University of Southampton, Highfield Campus, Southampton SO17 1BJ, UK.
11
Department of Plant Pathology, Faculty of Agriculture, University of Zagreb, Svetosimunska 25, 10000 Zagreb, Croatia.
12
Centre for Crop Health, University of Southern Queensland, Toowoomba QLD 4350, Australia.
13
Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462 Martonvasar, Hungary.
14
Institut Français de la Vigne et du Vin, Pôle Rhône-Méditerranée, 7 avenue Cazeaux, 30230 Rodilhan, France.
15
IRTA Centre de Cabrils, Carretera de Cabrils km 2, 08348 Cabrils, Spain.
16
Shamir Research Institute, Katsrin, Israel.
17
INIA-CIFOR, C. Coruna km 7.5, 28040 Madrid, Spain.
18
University of Debrecen, Institute of Food Science, 4032 Debrecen, Böszörményi út 138, Hungary.
19
Centre for Research and Development, Eszterházy Károly University, H-3300 Eger, Hungary.
20
Wageningen University and Research Centre (WUR), Laboratory of Phytopathology, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
21
Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, P. Bag X20, Pretoria 0028, South Africa.

Abstract

Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on grapevines, either as agents of pre- or post-harvest infections, including Phomopsis cane and leaf spot, cane bleaching, swelling arm and trunk cankers. In this study we explore the occurrence, diversity and pathogenicity of Diaporthe spp. associated with Vitis vinifera in major grape production areas of Europe and Israel, focusing on nurseries and vineyards. Surveys were conducted in Croatia, Czech Republic, France, Hungary, Israel, Italy, Spain and the UK. A total of 175 Diaporthe strains were isolated from asymptomatic and symptomatic shoots, branches and trunks. A multi-locus phylogeny was established based on five genomic loci (ITS, tef1, cal, his3 and tub2), and the morphological characters of the isolates were determined. Preliminary pathogenicity tests were performed on green grapevine shoots with representative isolates. The most commonly isolated species were D. eres and D. ampelina. Four new Diaporthe species described here as D. bohemiae, D. celeris, D. hispaniae and D. hungariae were found associated with affected vines. Pathogenicity tests revealed D. baccae, D. celeris, D. hispaniae and D. hungariae as pathogens of grapevines. No symptoms were caused by D. bohemiae. This study represents the first report of D. ambigua and D. baccae on grapevines in Europe. The present study improves our understanding of the species associated with several disease symptoms on V. vinifera plants, and provides useful information for effective disease management.

KEYWORDS:

Vitis; canker; multi-locus sequence typing; pathogenicity

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center