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Biochem Biophys Res Commun. 2019 Jan 8;508(2):361-367. doi: 10.1016/j.bbrc.2018.11.154. Epub 2018 Nov 28.

Ceramide kinase regulates the migration of bone marrow-derived mesenchymal stem cells.

Author information

1
Graduate School of Biotechnology, Kyung Hee University, Yongin, 17104, South Korea.
2
Department of Applied Chemistry, Kyung Hee University, Yongin, 17104, South Korea.
3
Department of Applied Chemistry, Kyung Hee University, Yongin, 17104, South Korea; Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul, 02447, South Korea; Department of New Biology, DGIST, Daegu, 42988, South Korea. Electronic address: mkim@dgist.ac.kr.
4
Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul, 02447, South Korea; East-West Medical Research Institute, Kyung Hee University, Seoul, 02447, South Korea; Kyung Hee University Medical Center, Seoul, 02447, South Korea. Electronic address: kisookpark@khu.ac.kr.

Abstract

Endogenous bone marrow-derived mesenchymal stem cells (BM-MSCs) are mobilized into peripheral blood and injured tissues by various growth factors and cytokines that are expressed in the injured tissues, such as substance P (SP), stromal cell derived factor-1 (SDF-1), and transforming growth factor-beta (TGF-β). Extracellular bioactive lipid metabolites such as ceramide-1-phosphate and sphingosine-1-phosphate also modulate BM-MSC migration as SP, SDF-1, and TGF-β. However, the roles of intrinsic lipid kinases of BM-MSCs in the stem cell migration are unclear. Here, we demonstrated that ceramide kinase mediates the chemotactic migration of BM-MSCs in response to SP, SDF-1, or TGF-β. Furthermore, a specific inhibitor of ceramide kinase inhibited TGF-β-induced migration of BM-MSCs and N-cadherin that is necessary for BM-MSCs migration in response to TGF-β. Therefore, these results suggest that the intracellular ceramide kinase is required for the BM-MSCs migration and the roles of the intrinsic ceramide kinase in the migration are associated with N-cadherin regulation.

KEYWORDS:

Ceramide kinase; Mesenchymal stem cells; Migration; N-cadherin; TGF-β

PMID:
30502084
DOI:
10.1016/j.bbrc.2018.11.154

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