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J Mol Biol. 1988 Aug 5;202(3):667-72.

Testing for intron function in the essential Saccharomyces cerevisiae tRNA(SerUCG) gene.

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Division of Chemistry, California Institute of Technology, Pasadena, CA 91125.


The gene sup61+, which codes for the essential Saccharomyces cerevisiae tRNA(SerUCG), is the only single-copy tRNA gene in this organism know to contain an intron. To assess the role of this intron in tRNA gene expression, an intron-deleted sup61+ gene was constructed in vitro and introduced into the yeast genome. Isogenic intron- and intron+ strains were found to be indistinguishable by criteria that include growth rates, ability to undergo meiosis, levels of mature tRNA(SerUCG) transcribed in vivo, and the suppressor efficiency of amber- and ochre-specific alleles of this gene.

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