Format

Send to

Choose Destination
Angew Chem Int Ed Engl. 2018 Nov 30. doi: 10.1002/anie.201811951. [Epub ahead of print]

Exploring the Fundamental Structures of Life: Non-targeted, Chemical Analysis of Single Cells and Subcellular Structures.

Author information

1
Department of Chemistry and the Beckman Institute for Advanced Science and Technology, 405 N. Mathews Avenue, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States, UNITED STATES.
2
Department of Chemistry, 1420 Circle Drive, University of Tennessee, Knoxville, Tennessee 37996, United States, UNITED STATES.
3
University of Illinois, Department of Chemistry, 600 S. Mathews Ave. 63-5, 61801-5004, Urbana, UNITED STATES.

Abstract

Cells are a basic functional and structural unit of living organisms. Both unicellular communities and multicellular species produce an astonishing chemical diversity, enabling a wide range of divergent functions, yet each cell shares numerous aspects that are common to all living organisms. While there are many approaches for studying this chemical diversity, only a few are non-targeted and capable of analyzing hundreds of different chemicals at cellular resolution. Here, we review the non-targeted approaches used to perform comprehensive chemical analyses, provide chemical imaging information, or obtain high-throughput single cell profiling data. Single cell measurement capabilities are rapidly increasing in terms of throughput, limits of detection, and completeness of the chemical analyses; these improvements enable their application to understand ever more complex physiological phenomena such as learning, memory, and behavior.

KEYWORDS:

Analytical methods; Bioanalysis; Mass Spectrometry; Single Cell Analysis; imaging

PMID:
30500998
DOI:
10.1002/anie.201811951

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center