Format

Send to

Choose Destination
Sci Rep. 2018 Nov 29;8(1):17461. doi: 10.1038/s41598-018-35802-9.

Glutamatergic and dopaminergic modulation of cortico-striatal circuits probed by dynamic calcium imaging of networks reconstructed in microfluidic chips.

Author information

1
CNRS UMR 8256, Biological Adaptation and Ageing, Paris, 75005, France.
2
Sorbonne Universités, UPMC, Institut de Biologie Paris-Seine, 75005, Paris, France.
3
CNRS UMR 8246, Neurosciences, Paris, 75005, France.
4
Servier Biotechnology, Chemogenetic Laboratory @ ICM Brain & Spine Institue, Pitié-Salpétrière Hospital, 52 boulevard Vincent Auriol, 75013, Paris, France.
5
Centre for Therapeutic Innovation in Neuropsychiatry, Institut de Recherches Servier, Centre de Recherches de Croissy, 78290, Croissy-sur-Seine, France.
6
CNRS UMR 8246, Neurosciences, Paris, 75005, France. jean-michel.peyrin@upmc.fr.
7
Sorbonne Universités, UPMC, Institut de Biologie Paris-Seine, 75005, Paris, France. jean-michel.peyrin@upmc.fr.

Abstract

Although the prefrontal cortex and basal ganglia are functionally interconnected by parallel loops, cellular substrates underlying their interaction remain poorly understood. One novel approach for addressing this issue is microfluidics, a methodology which recapitulates several intrinsic and synaptic properties of cortico-subcortical networks. We developed a microfluidic device where cortical neurons projected onto striatal neurons in a separate compartment. We exploited real-time (low-resolution/high-output) calcium imaging to register network dynamics and characterize the response to glutamatergic and dopaminergic agents. Reconstructed cortico-striatal networks revealed the progressive appearance of cortical VGLUT1 clusters on striatal dendrites, correlating with the emergence of spontaneous and synchronous glutamatergic responses of striatal neurons to concurrent cortical stimulation. Striatal exposure to the NMDA receptor GluN2A subunit antagonist TCN201 did not affect network rhythm, whereas the GluN2B subunit antagonist RO256981 significantly decreased striatal activity. Dopamine application or the D2/D3 receptor agonist, quinpirole, decreased cortico-striatal synchrony whereas the D1 receptor agonist, SKF38393, was ineffective. These data show that cortico-striatal networks reconstructed in a microfluidic environment are synchronized and present characteristics close to those of their in situ counterparts. They should prove instructive for deciphering the molecular substrates of CNS disorders and evaluating the actions of novel therapeutic agents.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center