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BMC Plant Biol. 2018 Nov 29;18(1):310. doi: 10.1186/s12870-018-1520-1.

Combined linkage and association mapping reveal QTL for host plant resistance to common rust (Puccinia sorghi) in tropical maize.

Zheng H1,2,3, Chen J3,4, Mu C3,5, Makumbi D6, Xu Y3,7, Mahuku G8.

Author information

1
CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China.
2
Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
3
International Maize and Wheat Improvement Center (CIMMYT), Apdo, Postal 6-641, 06600, Mexico, DF, Mexico.
4
College of Life Sciences, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, China.
5
Maize Research Institute, Shandong Agricultural Academy of Sciences, Jinan, China.
6
International Maize and Wheat Improvement Center (CIMMYT), P.O Box 1041-00621, Nairobi, Kenya.
7
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
8
International Institute of Tropical Agriculture (IITA), P.O. Box, 34443, Dar es Salaam, Tanzania. g.mahuku@cgiar.org.

Abstract

BACKGROUND:

Common rust, caused by Puccinia sorghi, is an important foliar disease of maize that has been associated with up to 50% grain yield loss. Development of resistant maize germplasm is the ideal strategy to combat P. sorghi.

RESULTS:

Association mapping performed using a mixed linear model (MLM), integrating population structure and family relatedness identified 25 QTL (P < 3.12 × 10- 5) that were associated with resistance to common rust and distributed on chromosomes 1, 3, 5, 6, 8, and 10. We identified three QTLs associated with all three disease parameters (final disease rating, mean disease rating, and area under disease progress curve) located on chromosomes 1, 3, and 8. A total of 5 QTLs for resistance to common rust were identified in the RIL population. Nine candidate genes located on chromosomes 1, 5, 6, 8, and 10 for resistance to common rust associated loci were identified through detailed annotation.

CONCLUSIONS:

Using a diverse set of inbred lines genotyped with high density markers and evaluated for common rust resistance in multiple environments, it was possible to identify QTL significantly associated with resistance to common rust and several candidate genes. The results point to the need for fine mapping common rust resistance by targeting regions identified in common between this study and others using diverse germplasm.

KEYWORDS:

Genome-wide association study, maize diseases; Puccinia sorghi; Single-nucleotide polymorphisms

PMID:
30497411
PMCID:
PMC6267831
DOI:
10.1186/s12870-018-1520-1
[Indexed for MEDLINE]
Free PMC Article

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