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Int J Infect Dis. 2018 Nov 26. pii: S1201-9712(18)34591-0. doi: 10.1016/j.ijid.2018.11.012. [Epub ahead of print]

New ST in multidrug-resistant Klebsiella pneumoniae harboring blaNDM-1 encoding gene in Brazil.

Author information

1
Departamento de Patologia, Análises Clínicas e Toxicológicas, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
2
School of Dentistry, University of Ribeirão Preto, Ribeirão Preto, SP, Brazil.
3
Departamento de Patologia, Análises Clínicas e Toxicológicas, Universidade Estadual de Londrina, Londrina, Paraná, Brazil. Electronic address: eliana.vespero@gmail.com.

Abstract

OBJECTIVES:

The aim of this study was to investigate the blaNDM gene, the pathogenic potential and antimicrobial resistance in clinical isolates of carbapenem-resistant Klebsiella pneumoniae isolated from patients admitted to the University Hospital of Londrina between January 2014 and March 2017.

METHODS:

blaNDM-1 and virulence genes were investigated using conventional PCR methods Antimicrobial susceptibility tests were performed the disk diffusion method according to the standards described in CLSI 2017. Clonal relationships the NDM-positive isolates were determined by ERIC-PCR and MLST.

RESULTS:

A total of 825K. pneumoniae were identified with four isolates (Kp6408, Kp6410, Kp6411 and Kp6715) presenting the blaNDM-1 gene. All NDM-1-producing isolates showed co-production of blaKPC-2 and blaTEM genes and also as well as the virulence genes kfu, entB, mrkD and fimH. Three isolates (Kp6408, Kp6410, Kp6411 and Kp6715) were classified as multidrug-resistant (MDR) and one (Kp6411) as extensively drug-resistant (XDR). Enterobacterial repetitive intergenic consensus (ERIC-PCR) analyses demonstrated that the isolates share about 60% genetic similarity. Multilocus sequence typing (MLST) revealed four different sequence types (STs), first described in Brazil, with two novel STs described in this study: ST3371 and ST3372.

CONCLUSION:

This study reports the identification of NDM-1 associated with KPC and virulence genes in four MDR K. pneumoniae with STs first described in Brazil.

KEYWORDS:

Klebsiella pneumoniae; MLST; NDM; carbapenemases

PMID:
30496852
DOI:
10.1016/j.ijid.2018.11.012
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