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Int J Radiat Biol. 2018 Nov 29:1-22. doi: 10.1080/09553002.2019.1542182. [Epub ahead of print]

Protective properties of Myrtus communis extract against oxidative effects of extremely low-frequency magnetic fields on rat plasma and hemoglobin.

Author information

1
a Arak University of Medical Sciences and Khansari Hospital, Department of Medical Physics and Radiotherapy , Arak , Iran.
2
b Arak University of Medical Sciences, Department of Biochemistry and Genetics , Arak , Iran.
3
c Arak University of Medical Sciences, Infectious Diseases Research Center , Arak , Iran.

Abstract

PURPOSE:

This study investigates the protective properties of Myrtus communis extract against oxidative effects of Extremely Low Frequency Magnetic Fields (ELFMF). Also this study is aimed to analyze the conformational changes of hemoglobin, oxidative damages to plasma proteins and antioxidant power of plasma following exposure to ELFMF.

MATERIALS AND METHODS:

Adult male rats were divided into 3 groups: (1) control, (2) ELFMF exposure, and (3) ELFMF exposure after Myrtus communis extract administration. The magnetic field (0.7 mT, 50 Hz) was produced by a Helmholtz coil for one month, 2 hours a day. The Myrtus communis extract was injected intraperitoneally at a dose of 0.5 mg/kg before exposure to ELFMF. The oxidative effects of ELFMF were studied by evaluating the hemoglobin, methemoglobin (metHb) and hemichrome levels, absorption spectrum of hemoglobin (200 to 700 nm), oxidative damage to plasma proteins by measuring protein carbonyl (PCO) levels and plasma antioxidant power according to ferric reducing ability of plasma (FRAP). The mean and standard errors of mean were determined for each group. One-way ANOVA analysis was used to compare the means of groups. The significance level was considered to be P < 0.05. Moreover, artificial neural network (ANN) analysis was used to identify the predictive parameters for estimating the oxyhemoglobin (oxyHb) concentration.

RESULTS:

Exposure to ELFMF decreased the FRAP which was in concomitant with a significant increase in plasma PCO, metHb and hemichrome concentrations (p < 0.001). Oxidative modifications of Hb were shown by reduction in optical density at 340nm (globin-heme interaction) and 420 nm (heme-heme interaction). Administration of Myrtus communis extract increased FRAP values and decreased plasma POC, metHb and hemichrome concentrations. Also a significant increase in Hb absorbance at 340, 420, 542 and 577 nm showed the protective properties of Myrtus communis extract against ELFMF-induced oxidative stress in erythrocytes. ANN analysis showed that optical absorption of hemoglobin at 520, 577, 542, and 630 nm and concentration of metHb and hemichrome were the most important parameters in predicting the oxyHb concentration.

CONCLUSIONS:

Myrtus communis extract enhances the ability of erythrocytes and plasma to deal with oxidative conditions during exposure to ELFMF. Also ANN analysis can predict the most important parameters in relation to Hb structure during oxidative stress.

KEYWORDS:

Extremely Low Frequency Magnetic Fields; Myrtus communis; hemoglobin; oxidative stress; plasma

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