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J Nat Prod. 2018 Dec 28;81(12):2750-2755. doi: 10.1021/acs.jnatprod.8b00761. Epub 2018 Nov 29.

Harnessing Natural Product Diversity for Fluorophore Discovery: Naturally Occurring Fluorescent Hydroxyanthraquinones from the Marine Crinoid Pterometra venusta.

Author information

1
Molecular Targets Program, Center for Cancer Research , National Cancer Institute , Frederick , Maryland 21702-1201 , United States.
2
Chemical Biology Laboratory, Center for Cancer Research , National Cancer Institute , Frederick , Maryland 21702-1201 , United States.
3
Basic Science Program, Leidos Biomedical Research, Inc. , Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute , Frederick , Maryland 21702-1201 , United States.
4
Natural Products Branch, Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis , National Cancer Institute , Frederick , Maryland 21701-1201 , United States.

Abstract

Fluorescent small molecules are important tools in many aspects of modern biology. A two-stage evaluation process involving fluorescence screening and live-cell imaging was developed to facilitate the identification of new fluorescent probes from extracts housed within the NCI Natural Products Repository. To this end, over 2000 extracts and prefractionated samples were examined, including an extract from the marine crinoid Pterometra venusta. An optically guided evaluation involving stepwise fluorescence screening and live-cell imaging was developed to enable the isolation of fluorescent natural products. These efforts resulted in the isolation of six hydroxyanthraquinone compounds, three of which are new natural products. These purified metabolites were examined for their potential as cellular imaging probes, and they demonstrate that natural product libraries can be a good source of new fluorescent agents.

PMID:
30495954
PMCID:
PMC6474788
DOI:
10.1021/acs.jnatprod.8b00761
[Indexed for MEDLINE]
Free PMC Article

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