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Mol Cells. 2018 Nov 30;41(11):943-952. doi: 10.14348/molcells.2018.0408. Epub 2018 Nov 14.

CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes.

Author information

1
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Korea.
2
Department of Pathology, College of Medicine, Kyung Hee University, Seoul 02447, Korea.

Abstract

The discovery and mechanistic understanding of target-specific genome engineering technologies has led to extremely effective and specific genome editing in higher organisms. Target-specific genetic modification technology is expected to have a leading position in future gene therapy development, and has a ripple effect on various basic and applied studies. However, several problems remain and hinder efficient and specific editing of target genomic loci. The issues are particularly critical in precise targeted insertion of external DNA sequences into genomes. Here, we discuss some recent efforts to overcome such problems and present a perspective of future genome editing technologies.

KEYWORDS:

CRISPR; DNA double-strand break; genome engineering; knock-in; target specific endonuclease

PMID:
30486613
PMCID:
PMC6277560
DOI:
10.14348/molcells.2018.0408
[Indexed for MEDLINE]
Free PMC Article

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