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Zhonghua Yi Xue Za Zhi. 2018 Nov 27;98(44):3607-3612. doi: 10.3760/cma.j.issn.0376-2491.2018.44.012.

[Performance of matrix assisted laser desorption ionization time of flight biotyper system in clinical bacteria identification].

[Article in Chinese; Abstract available in Chinese from the publisher]

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Department of Clinical Laboratory of the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, China.


in English, Chinese

Objective: To evaluate the performance of MALDI Biotyper system in identification of clinically isolated pathogens so as to provide a new rapid identification method. Methods: Total 21 270 pathogens strains, isolated from the First Affiliated Hospital of Fujian Medical Universityduring Nov. 2015 to Dec. 2016, were identified by VITEK-Ⅱ, API and MALDI Biotyper system, respectively.The isolated strains were confirmed by DNA sequencing. Results: The identification of common bacteria with MALDI Biotyper and phenotypic system is highly consistent (>95% and >90%). Among 43 strains of anaerobic bacteria, MALDI Biotyper could identify 90.7% bacteria to species level and 97.7% bacteria to genus level with the statistical significance(χ(2)=6.76, P<0.01), while phenotypic system only identified 65.1% bacteria to species and 69.8% bacteria to genus. Also, no statistical significance was shown for Trichosporon and Candida(P>0.05). MALDI Biotyper could identify 76% filamentous fungi and all of Actinomycetes, Nocardia, Mycobacterium and Legionella to genus level. Conclusions: MALDI Biotyper is an easy-performed, sensitive method for the identification of clinically isolated pathogens. Additionally, the pretreatment and reference database has the effect on identification.


Bacteria; Biological assay; Spectrometry, mass, matrix-assisted laser desorption-ionization


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