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J Appl Toxicol. 2018 Nov 28. doi: 10.1002/jat.3754. [Epub ahead of print]

In vitro genotoxicity assessment of an oxidized dextrin-based hydrogel for biomedical applications.

Author information

1
CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
2
Departamento de Saúde Ambiental, Instituto Nacional de Saúde Dr. Ricardo Jorge, 4000-055, Porto, Portugal.
3
EPIUnit-Instituto de Saúde Pública, Universidade do Porto, 4050-600, Porto, Portugal.

Abstract

Hydrogels are three-dimensional, crosslinked networks of hydrophilic polymers swollen with a large amount of water or biological fluids, without dissolving. Dextrin, a low-molecular-weight carbohydrate composed by glucose residues, has been used to develop an injectable hydrogel for biomedical applications. Dextrin was first oxidized to introduce aldehyde groups, which then reticulate with adipic acid dihydrazide, forming the dextrin-based hydrogel (HG). The HG and its components were tested for cyto- and genotoxicity according to the International Standard ISO 10993-3 on the biological evaluation of medical devices. To assess genotoxicity, a battery of in vitro genotoxicity tests employing both eukaryotic and prokaryotic models was performed: comet assay, cytokinesis-block micronucleus assay and Ames test. Our data revealed that the HG (IC50  = 2.8 mg/mL) and oxidized dextrin by itself (IC50  = 1.2 mg/mL) caused a concentration-dependent decrease in cellular viability of human lymphoblastoid TK6 cells after 24 hours of exposure to the test agents. However, these concentrations are unlikely to be reached in vivo. In addition, no significant increase in the DNA and chromosomal damage of TK6 cells exposed to non-cytotoxic concentrations of the HG and its isolated components was detected. Furthermore, neither the HG nor its metabolites exerted a mutagenic effect in different of Salmonella typhimurium strains and in an Escherichia coli mix. Our data demonstrated the genocompatibility of the HG (up to 3.5 mg/mL) for biomedical applications. To our best acknowledge, this is the first report with a detailed genotoxicity assessment of an aldehyde-modified polysaccharide/adipic acid dihydrazide hydrogel.

KEYWORDS:

Ames test; TK6 cells; adipic acid dihydrazide (ADH); aldehyde-modified polysaccharide; comet assay; cytokinesis-block micronucleus (CBMN) assay; dextrin; hydrogel

PMID:
30485472
DOI:
10.1002/jat.3754

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