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Food Sci Biotechnol. 2018 Jun 14;27(6):1755-1760. doi: 10.1007/s10068-018-0417-y. eCollection 2018 Dec.

Analysis of Leuconostoc citreum strains using multilocus sequence typing.

Author information

1
Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 South Korea.

Abstract

The objective of this study was to perform genetic diversity analysis of 13 strains isolated from South Korean foods by multilocus sequence typing (MLST). For typing, seven housekeeping loci (atpA, dnaA, dnaK, gyrB, pheS, pyrG, and rpoA) were selected, amplified and analyzed. Fifty-one polymorphic sites varying from 1 to 22 in each species were identified. Thirteen sequence types were generated with allele numbers ranged from 2 to 10. The overall relationship between strains was assessed by unweighted pair group method with arithmetic mean dendrogram and minimum spanning tree. In addition, combined spits tree analysis revealed intragenic recombination. No clear relationship was observed between the isolation sources and strains. The developed MLST scheme enhanced our knowledge of the population diversity of Leu. citreum strains and will be used further for the selection of industrially important strain.

KEYWORDS:

Alleles; Lactic acid bacteria; Leuconostoc citreum; MLST; Sequence type

PMID:
30483440
PMCID:
PMC6233419
[Available on 2019-06-14]
DOI:
10.1007/s10068-018-0417-y

Conflict of interest statement

Compliance with ethical standardsThe authors declare no conflict of interest.

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