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J Vet Sci. 2018 Nov 26. [Epub ahead of print]

Genetic identification of astroviruses in wild boars.

Author information

1
University of Veterinary Medicine and Pharmacy, Komensky 73, SK-041 81 Kosice Slovakia.

Abstract

Astroviruses were widely detected in pigs but their detection in wild boars is rather sporadic. In this study astroviruses were detected in organ homogenates of wild boars by a nested RT-PCR and the typing was carried out by phylogenetic analysis. Overall, 30/200 (15%) homogenates were positive for astroviruses. Genetic typing revealed that of 13 amplicons analysed, 8 were typed as porcine astrovirus lineage 2 (PAstV-2), and 2 as lineage 4 (PAstV-4). Two identical sequences were grouped with chicken astrovirus, and 1 sequence belonged to bat astrovirus. The first identification of chicken and bat astroviruses in wild boars indicates interspecies transmission.

KEYWORDS:

bat astrovirus; chicken astrovirus; phylogenetic analysis; porcine astrovirus; wild boar

PMID:
30481990

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