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PLoS One. 2018 Nov 27;13(11):e0208081. doi: 10.1371/journal.pone.0208081. eCollection 2018.

Percentage fractions of urinary di(2-ethylhexyl) phthalate metabolites: Association with obesity and insulin resistance in Korean girls.

Author information

1
Department of Pediatrics, Inje University Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Republic of Korea.
2
Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea.
3
Department of Chemistry, Korea University, Seoul, Republic of Korea.
4
Department of Internal Medicine, Inje University Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Republic of Korea.
5
Institute for Environmental Research, Yonsei University College of Medicine, Seoul, Republic of Korea.

Abstract

OBJECTIVE:

We assessed the associations of percentage fractions of urinary di(2-ethylhexyl) phthalate (DEHP) metabolites with obesity and insulin resistance in Korean girls.

METHODS:

In total, 137 girls, aged 6 to 13 years (65 overweight cases and 72 controls), were recruited. Anthropometric indices and the homeostatic model assessment of insulin resistance (HOMA-IR) index were determined. Four major urinary DEHP metabolites were analyzed in spot urine samples by gas chromatography-tandem mass spectrometry, including mono(2-ethylhexyl) phthalate, mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP), and mono(2-ethyl-5-carboxypentyl) phthalate.

RESULTS:

There were no significant differences in the urinary concentrations of the DEHP metabolites between the overweight and control groups. The percentage fraction of MEHHP (MEHHP%) among all DEHP metabolites was significantly higher in the overweight prepubertal girls than in the controls (P = 0.035). MEHHP% was positively associated with the body mass index percentile, waist circumference, body fat percentage, and HOMA-IR index in the prepubertal girls. After adjusting for covariates, the prepubertal girls in a higher MEHHP% quartile were found to have a higher odds ratio for central obesity than those in a lower quartile (odds ratios: 5.05 for quartile 3; 7.30 for quartile 4). The relative rate of MEHHP oxidation to MEOHP was negatively associated with the body mass index percentile and waist circumference in the prepubertal girls. However, no such association was observed in the pubertal girls.

CONCLUSIONS:

MEHHP% was positively associated with obesity and insulin resistance in prepubertal girls. Further studies are necessary to elucidate the causal links between altered phthalate metabolism and increased susceptibility to insulin resistance in children.

PMID:
30481198
PMCID:
PMC6258563
DOI:
10.1371/journal.pone.0208081
[Indexed for MEDLINE]
Free PMC Article

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