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J Proteome Res. 2019 Jan 4;18(1):120-129. doi: 10.1021/acs.jproteome.8b00391. Epub 2018 Dec 11.

200+ Protein Concentrations in Healthy Human Blood Plasma: Targeted Quantitative SRM SIS Screening of Chromosomes 18, 13, Y, and the Mitochondrial Chromosome Encoded Proteome.

Author information

1
Institute of Biomedical Chemistry , Moscow 119435 , Russia.
2
Pirogov Russian National Research Medical University , Moscow 117997 , Russia.
3
Institute of Medico-Biological Problems , Moscow 123007 , Russia.
4
Yonsei Proteome Research Center , Yonsei University , Seoul 03722 , Korea.
5
Department of Molecular Systems Biology, Cell Science Research Center , Royan Institute for Stem Cell Biology and Technology, ACECR , Tehran , Iran.
6
Department of Molecular Sciences , Macquarie University , Sydney , New South Wales 2109 , Australia.
7
Department of Systems Biology , Agricultural Biotechnology Research Institute of Iran , Karaj , Iran.
8
Area of Diagnostic Laboratories , Fondazione Policlinico Gemelli-IRCCS , Rome 00168 , Italy.
9
Institute of Biochemistry and Clinical Biochemistry , Catholic University of the Sacred Heart , Rome 00168 , Italy.

Abstract

This work continues the series of the quantitative measurements of the proteins encoded by different chromosomes in the blood plasma of a healthy person. Selected Reaction Monitoring with Stable Isotope-labeled peptide Standards (SRM SIS) and a gene-centric approach, which is the basis for the implementation of the international Chromosome-centric Human Proteome Project (C-HPP), were applied for the quantitative measurement of proteins in human blood plasma. Analyses were carried out in the frame of C-HPP for each protein-coding gene of the four human chromosomes: 18, 13, Y, and mitochondrial. Concentrations of proteins encoded by 667 genes were measured in 54 blood plasma samples of the volunteers, whose health conditions were consistent with requirements for astronauts. The gene list included 276, 329, 47, and 15 genes of chromosomes 18, 13, Y, and the mitochondrial chromosome, respectively. This paper does not make claims about the detection of missing proteins. Only 205 proteins (30.7%) were detected in the samples. Of them, 84, 106, 10, and 5 belonged to chromosomes 18, 13, and Y and the mitochondrial chromosome, respectively. Each detected protein was found in at least one of the samples analyzed. The SRM SIS raw data are available in the ProteomeXchange repository (PXD004374, PASS01192).

KEYWORDS:

Chromosome-centric Human Proteome Project (C-HPP); Human Plasma Proteome Project (HPPP); healthy human; plasma proteome; selected reaction monitoring (SRM SIS); targeted proteomics

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