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Nucleic Acids Res. 1988 Aug 25;16(16):7843-53.

Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein.

Author information

1
Friedrich Miescher Institut, Basel, Switzerland.

Abstract

The binding of the mutS gene product, a protein involved in at least two E. coli mismatch correction pathways, to a series of synthetic DNA duplexes containing mismatches or mismatch analogues of the purine/pyrimidine type was studied in order to establish whether a correlation exists between the recognition of these mispairs and the efficiency of their correction in vivo. Experiments using nitrocellulose filter binding or band-shift assays revealed that duplexes containing a G/T mismatch or its analogues I/T and DI/T were bound by the protein with affinities correlating to the efficiency of their repair in vivo. In contrast, the A/C mismatch, contained within the same sequence, was bound only poorly, despite being efficiently corrected in vivo. The analogues of the A/C mispair, uncorrected in vivo, were not detectably bound under the conditions of these assays.

PMID:
3047673
PMCID:
PMC338495
DOI:
10.1093/nar/16.16.7843
[Indexed for MEDLINE]
Free PMC Article

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