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Curr Opin Chem Biol. 2019 Feb;48:64-72. doi: 10.1016/j.cbpa.2018.10.023. Epub 2018 Nov 23.

Protein identification strategies in MALDI imaging mass spectrometry: a brief review.

Author information

1
Department of Chemistry, Vanderbilt University, 7330 Stevenson Center, Station B 351822, Nashville, TN 37235, USA; Mass Spectrometry Research Center, Vanderbilt University, 465 21st Ave S #9160, Nashville, TN 37235, USA.
2
Department of Chemistry, Vanderbilt University, 7330 Stevenson Center, Station B 351822, Nashville, TN 37235, USA; Mass Spectrometry Research Center, Vanderbilt University, 465 21st Ave S #9160, Nashville, TN 37235, USA; Department of Biochemistry, Vanderbilt University, 607 Light Hall, Nashville, TN 37205, USA.
3
Department of Chemistry, Vanderbilt University, 7330 Stevenson Center, Station B 351822, Nashville, TN 37235, USA; Mass Spectrometry Research Center, Vanderbilt University, 465 21st Ave S #9160, Nashville, TN 37235, USA; Department of Biochemistry, Vanderbilt University, 607 Light Hall, Nashville, TN 37205, USA; Department of Pharmacology, Vanderbilt University, 442 Robinson Research Building, 2220 Pierce Avenue, Nashville, TN 37232, USA; Department of Medicine, Vanderbilt University, 465 21st Ave S #9160, Nashville, TN 37235, USA. Electronic address: richard.m.caprioli@Vanderbilt.Edu.

Abstract

Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a powerful technology used to investigate the spatial distributions of thousands of molecules throughout a tissue section from a single experiment. As proteins represent an important group of functional molecules in tissue and cells, the imaging of proteins has been an important point of focus in the development of IMS technologies and methods. Protein identification is crucial for the biological contextualization of molecular imaging data. However, gas-phase fragmentation efficiency of MALDI generated proteins presents significant challenges, making protein identification directly from tissue difficult. This review highlights methods and technologies specifically related to protein identification that have been developed to overcome these challenges in MALDI IMS experiments.

PMID:
30476689
PMCID:
PMC6382520
[Available on 2020-02-01]
DOI:
10.1016/j.cbpa.2018.10.023
[Indexed for MEDLINE]

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