Paracoccidioides brasiliensis 14-3-3 protein is important for virulence in a murine model

Med Mycol. 2019 Oct 1;57(7):900-904. doi: 10.1093/mmy/myy112.

Abstract

The Paracoccidioides brasiliensis strain downregulated the expression of adhesin Pb14-3-3 (Pb14-3-3 aRNA) was evaluated in a murine model of paracoccidioidomycosis (PCM). Pb14-3-3 aRNA displays attenuated virulence and triggered the formation of fewer granulomas by lowering the fungal burden in the lungs. Additionally, the Pb14-3-3 aRNA showed more elongated yeast cells and less ability to induce pneumocytes apoptosis in vitro. Our results show that 14-3-3 is an important virulence factor in P. brasiliensis-induced pulmonary infection.

Keywords: Paracoccidioides brasiliensis: 14-3-3 protein; pneumocytes apoptosis; virulence in murine model.

MeSH terms

  • 14-3-3 Proteins / genetics*
  • Alveolar Epithelial Cells / microbiology
  • Alveolar Epithelial Cells / pathology
  • Animals
  • Apoptosis / genetics
  • Disease Models, Animal
  • Fungal Proteins / genetics*
  • Gene Expression
  • Lung / cytology
  • Lung / microbiology
  • Mice
  • Mice, Inbred BALB C
  • Paracoccidioides / genetics*
  • Paracoccidioides / pathogenicity*
  • Paracoccidioidomycosis / microbiology
  • Virulence Factors / genetics*

Substances

  • 14-3-3 Proteins
  • Fungal Proteins
  • Virulence Factors