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J Immunol Methods. 1988 Sep 13;112(2):201-5.

Malaria sporozoite penetration. A new approach by double staining.

Author information

1
INSERM U 313 et Département de Parasitologie, Groupe Hospitalier Pitié-Salpétrière, Paris, France.

Abstract

To determine, whether a sporozoite is outside the hepatocyte membrane or internalized, a double staining test was carried out using, successively, antibody labeled with peroxidase and fluorescein. This test permits the quantification of sporozoite entry and outline sporozoite-hepatocyte interactions.

PMID:
3047262
DOI:
10.1016/0022-1759(88)90358-4
[Indexed for MEDLINE]

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