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Int J Environ Res Public Health. 2018 Nov 22;15(12). pii: E2616. doi: 10.3390/ijerph15122616.

Urinary 8-Hydroxy-2'-deoxyguanosine (8-OHdG) Concentrations and Menstrual Cycle Characteristics in Female University Students.

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Department of Human Ecology, School of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Department of Anthropology, University of Washington Box 353100 Seattle, Washington 98195, DC, USA.
Faculty of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura, Ora, Gunma 374-0113, Japan.
National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba 305-8506, Japan.
Department of Environment Systems, Graduate School of Frontier Science, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
Institute of Environmental Ecology, IDEA Consultants, Inc., 1334-5 Riemon, Yaizu, Shizuoka 421-0212, Japan.
Institute of Environmental Ecology, IDEA Consultants, Inc., 1334-5 Riemon, Yaizu, Shizuoka 421-0212, Japan.
Faculty of Nursing, Tokyo Healthcare University, 2-5-1 Higashigaoka, Meguro, Tokyo 152-8558, Japan.


Higher concentrations of oxidative stress biomarkers are found in women with polycystic ovary syndrome (PCOS) and endometriosis, conditions linked to irregular menstrual cycles and menstrual pain. The aim of the present study was to test whether women with higher oxidative stress are more likely to show irregular menstrual cycles and severe menstrual pain compared with women with lower oxidative stress. A cross-sectional study was conducted targeting female university students with a mean (SD) age of 20.5 (1.8) years (n = 188). Participants completed a questionnaire on reproductive characteristics and anthropometry and kept a menstrual cycle diary for 5 consecutive months. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), cotinine, and creatinine concentrations were measured once during the study period. The mean (SD) value of the urinary 8-OHdG concentration was 4.7 (2.0) μg/g of creatinine. A total of 1021 menstrual cycles were recorded. The participants were categorized as either having regular (68%) or irregular (18%) cycles or oligomenorrhea (13%) or polymenorrhea (1%). The urinary 8-OHdG concentration did not significantly differ across menstrual cycle regularity or pain categories. Even after adjusting for age, body mass index (BMI), and urinary cotinine concentrations, having irregular cycles or more severe menstrual pain was not associated with urinary 8-OHdG concentration.


menstrual cycle regularity; menstrual pain; oxidative stress; young women

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