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Diabetes Metab Res Rev. 2019 Feb;35(2):e3104. doi: 10.1002/dmrr.3104. Epub 2018 Dec 19.

MicroRNA-296-5p promotes healing of diabetic wound by targeting sodium-glucose transporter 2 (SGLT2).

Author information

1
School of Environmental Science and Engineering, Shanghai University, Shanghai, China.
2
Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai, China.
3
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Abstract

BACKGROUND:

Diabetic wounds are refractory and very difficult to heal. We aimed to use miRNA to identify novel and specific molecular markers for diabetes mellitus (DM) diagnosis and treatment.

METHODS:

The expression level of miR-296-5p was determined in tissue samples of 12 DM patients. The effect of miR-296-5p on proliferation of β-cells was examined using Cell Counting Kit-8 (CCK-8) and colony formation assay. The effect of miR-296-5p on cell cycle progression was analysed using flow cytometry. The target gene was verified using luciferase reporter assay. A rat diabetes model was used to assess the effect of miR-296-5p in vivo.

RESULTS:

Overexpression of miR-296-5p suppressed cell proliferation, arrested cell cycle progression, and increased the healing rate of diabetic wounds both in vivo and in vitro. TargetScan analysis results showed that miR-296-5p is a direct regulator of SGLT2.

CONCLUSIONS:

miR-296-5p can increase the healing rate of diabetic wounds and may be an effective molecular tool in DM diagnosis and therapy.

KEYWORDS:

SGLT2; cell cycle; diabetes; miR-296-5p; proliferation; wound healing

PMID:
30467970
DOI:
10.1002/dmrr.3104

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