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Science. 2018 Nov 23;362(6417):918-922. doi: 10.1126/science.aat6457.

Neutral mass spectrometry of virus capsids above 100 megadaltons with nanomechanical resonators.

Author information

1
Université Grenoble Alpes, F-38000 Grenoble, France.
2
CEA, BIG, Biologie à Grande Echelle, F-38054 Grenoble, France.
3
Inserm, Unité 1038, F-38054 Grenoble, France.
4
Université Grenoble Alpes, CEA, LETI, 38000 Grenoble, France.
5
Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif sur Yvette cedex, France.
6
Université Grenoble Alpes, F-38000 Grenoble, France. christophe.masselon@cea.fr sebastien.hentz@cea.fr.
7
Université Grenoble Alpes, CEA, LETI, 38000 Grenoble, France. christophe.masselon@cea.fr sebastien.hentz@cea.fr.

Abstract

Measurement of the mass of particles in the mega- to gigadalton range is challenging with conventional mass spectrometry. Although this mass range appears optimal for nanomechanical resonators, nanomechanical mass spectrometers often suffer from prohibitive sample loss, extended analysis time, or inadequate resolution. We report on a system architecture combining nebulization of the analytes from solution, their efficient transfer and focusing without relying on electromagnetic fields, and the mass measurements of individual particles using nanomechanical resonator arrays. This system determined the mass distribution of ~30-megadalton polystyrene nanoparticles with high detection efficiency and effectively performed molecular mass measurements of empty or DNA-filled bacteriophage T5 capsids with masses up to 105 megadaltons using less than 1 picomole of sample and with an instrument resolution above 100.

PMID:
30467165
DOI:
10.1126/science.aat6457
[Indexed for MEDLINE]

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