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Int J Biol Macromol. 2019 Feb 15;123:923-930. doi: 10.1016/j.ijbiomac.2018.11.187. Epub 2018 Nov 19.

Immune-enhancing effects of polysaccharides from purple sweet potato.

Author information

1
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.
2
College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu, China; Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai Area, Xuzhou 221131, Jiangsu, China.
3
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China. Electronic address: junliu@yzu.edu.cn.
4
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China. Electronic address: chjin@yzu.edu.cn.

Abstract

Immune-enhancing effects of three kinds of purple sweet potato polysaccharides (PSPPs) including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were evaluated. Scanning electron microscope analysis showed that all PSPPs could stimulate the formation of microvilli-like structures in cellular surfaces, which was possibly related to activation of macrophages. Neutral red uptake assay showed that PSPPs could increase the phagocytic activity of cells. High dose (400 μg/mL) of PSPPs could notably augment the level of nitric oxide (NO). ELISA analysis revealed that 200 and 400 μg/mL of PSPPs distinctly elevated the production of IL-1β. Cells received 200 and 400 μg/mL of WSP as well as 400 μg/mL of DASP exhibited higher level of IL-6. Results of animal experiments showed that WSP treatment (400 mg/kg) could promote the secretions of IgA, IgG, IgM and sIgA in both normal and immunosuppressed mice. Moreover, CASP treatment (400 mg/kg) elevated the production of IgM in the serum of normal and immunosuppressive mice, while DASP (400 mg/kg) only improved the secretion of IgM in normal mice. In summary, all three polysaccharides can stimulate immune responses of macrophages and positively regulate adaptive immunity by enhancing the production of immunoglobulins in mice.

KEYWORDS:

Immune-enhancing effects; Immunoglobulins; Macrophages; Polysaccharides; Purple sweet potato

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