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Biol Trace Elem Res. 2019 Jul;190(1):172-186. doi: 10.1007/s12011-018-1533-5. Epub 2018 Nov 22.

Neural Differentiation of Mouse Neural Stem Cells as a Tool to Assess Developmental Neurotoxicity of Drinking Water in Taihu Lake.

Author information

1
Stem Cell Translational Research Center, Tongji Hospital, School of Life Science and Technology, Tongji University, Shanghai, 200065, People's Republic of China.
2
Department of Regenerative Medicine, Tongji University School of Medicine, 1239 Siping Road, Shanghai, 200092, People's Republic of China.
3
College of Environmental Science and Engineering, Tongji University, Key Laboratory of Yangtze River Water Environment, Ministry of Education, Shanghai, 200092, People's Republic of China.
4
Department of Regenerative Medicine, Tongji University School of Medicine, 1239 Siping Road, Shanghai, 200092, People's Republic of China. junzhang@tongji.edu.cn.
5
Stem Cell Translational Research Center, Tongji Hospital, School of Life Science and Technology, Tongji University, Shanghai, 200065, People's Republic of China. zhangjing@tongji.edu.cn.
6
Tongji Hospital, School of Life Science and Technology, Tongji University, 389 Xincun Road, 200065, Shanghai, People's Republic of China. zhangjing@tongji.edu.cn.

Abstract

In this study, we used neural stem cells (NSCs) as a toxicology tool to assess the potential developmental neurotoxicity of drinking water from Taihu Lake. We found that the condensed drinking water could inhibit the proliferation and differentiation of NSCs, especially the tap water. Inductively coupled plasma mass spectrometry and high-performance liquid chromatography analysis showed that nickel was detected in the tap water with a high concentration. Our study revealed that nickel could inhibit NSCs proliferation and differentiation, which is induced not only by the intracellular reactive oxygen species generation, but also by the protein levels upregulation of p-c-Raf, p-MEK1/2 and p-Erk1/2 through the axon guidance signal pathways. These findings will provide a new way of research insight for investigation of nickel-induced neurotoxicity. Meanwhile, our test method confirmed the feasibility and reliability of stem cell assays for developmental neurotoxicity testing.

KEYWORDS:

Developmental neurotoxicity testing; Drinking water; Neural stem cells

PMID:
30465171
DOI:
10.1007/s12011-018-1533-5

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