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J Microsc Ultrastruct. 2018 Oct-Dec;6(4):182-187. doi: 10.4103/JMAU.JMAU_3_18.

Preparation of Sliver and Selenium Nanoparticles and Its Characterization by Dynamic Light Scattering and Scanning Electron Microscopy.

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Department of Biotechnology, IFTM University, Moradabad, Uttar Pradesh, India.
Department of Bio Science and Technology, Shri Ramswaroop Memorial University, Lucknow-Deva Road, Lucknow, Uttar Pradesh, India.



In our study, two different methods were used to determine the size and size distribution of the sliver and selenium nanoparticles via dynamic light scattering (DLS) and scanning electron microscopy (SEM).


Nanotechnology dealing with metal and metalloid nanoparticles has been usually applied in nearly each field of science, engineering, and technology including biology and medicine etc due to presence of size and shape dependent unusual physical and chemical properties. In the most recent decade, numerous groups including appreciably developed metal and metalloid nanoparticles based theranostic approaches for the treatment of almost human diseases. Amongst many nanoparticles, recently silver and selenium nanoparticles have been broadly used in the antimicrobial coatings, textiles, paints, keyboards, engineering, food industry, electronics, cosmetics, bio-sensing, wound dressings, and even in biomedical devices.


In our study, silver nanoparticles were prepared by using the chemical reduction method. Selenium nanoparticles (SeNPs) were synthesized by the chemical reduction of sodium selenite by glutathione (reduced form) and stabilized by bovine serum albumin (BSA). Characterization of silver and selenium nanoparticles samples were analyzed by dynamic light scattering (DLS) and Scanning Electron Microscopy (SEM).


Due to characterization by DLS technique, nanoparticles size was found the range of 79.22 nm and 178 nm for Sliver and Selenium Nanoparticles respectively. Sliver nanoparticles shown morphological average size and shape with SEM reveals spherical shape particles with the size of 80.32 nm whereas Selenium nanoparticles shown rod shape particles with the size of 74.29 nm.


Biomedical devices; dynamic light scattering; microbial infections; nanotechnology; scanning electron microscopy; selenium nanoparticles

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