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Pharmaceuticals (Basel). 2018 Nov 20;11(4). pii: E126. doi: 10.3390/ph11040126.

Effects of Boswellia Serrata Roxb. and Curcuma longa L. in an In Vitro Intestinal Inflammation Model Using Immune Cells and Caco-2.

Author information

1
Department of Physical Sciences, Hearth and Environment, University of Siena, Via Laterina 8, 53100 Siena, Italy. paolo.governa@unisi.it.
2
Department of Biotechnology, Chemistry and Pharmacy⁻Department of Excellence 2018⁻2022, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy. paolo.governa@unisi.it.
3
Department of Physical Sciences, Hearth and Environment, University of Siena, Via Laterina 8, 53100 Siena, Italy. maddimar@hotmail.com.
4
Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Largo Egidio Meneghetti 2, 35131 Padua, Italy. veronica.cocetta@gmail.com.
5
MRC Centres for Inflammation Research and Reproductive Health, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK. s1263113@ed-alumni.net.
6
MRC Centre for Inflammation Research, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK. p.saunders@ed.ac.uk.
7
Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Largo Egidio Meneghetti 2, 35131 Padua, Italy. daniela.catanzaro@unipd.it.
8
Department of Physical Sciences, Hearth and Environment, University of Siena, Via Laterina 8, 53100 Siena, Italy. miraldi@unisi.it.
9
Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Largo Egidio Meneghetti 2, 35131 Padua, Italy. monica.montopoli@unipd.it.
10
Venetian Institute of Molecular Medicine, Via Orus 2, 35129 Padua, Italy. monica.montopoli@unipd.it.
11
Department of Physical Sciences, Hearth and Environment, University of Siena, Via Laterina 8, 53100 Siena, Italy. biagi4@unisi.it.

Abstract

Inflammatory bowel diseases, which consist of chronic inflammatory conditions of the colon and the small intestine, are considered a global disease of our modern society. Recently, the interest toward the use of herbal therapies for the management of inflammatory bowel diseases has increased because of their effectiveness and favourable safety profile, compared to conventional drugs. Boswellia serrata Roxb. and Curcuma longa L. are amongst the most promising herbal drugs, however, their clinical use in inflammatory bowel diseases is limited and little is known on their mechanism of action. The aim of this work was to investigate the effects of two phytochemically characterized extracts of B. serrata and C. longa in an in vitro model of intestinal inflammation. Their impact on cytokine release and reactive oxygen species production, as well as the maintenance of the intestinal barrier function and on intestinal mucosa immune cells infiltration, has been evaluated. The extracts showed a good protective effect on the intestinal epithelium at 1 µg/mL, with TEER values increasing by approximately 1.5 fold, compared to LPS-stimulated cells. C. longa showed an anti-inflammatory mechanism of action, reducing IL-8, TNF-α and IL-6 production by approximately 30%, 25% and 40%, respectively, compared to the inflammatory stimuli. B. serrata action was linked to its antioxidant effect, with ROS production being reduced by 25%, compared to H₂O₂-stimulated Caco-2 cells. C. longa and B. serrata resulted to be promising agents for the management of inflammatory bowel diseases by modulating in vitro parameters which have been identified in the clinical conditions.

KEYWORDS:

Boswellia serrata Roxb.; Caco-2; Curcuma longa L.; HMC-1.1; PBMC; cytokines; intestinal bowel diseases (IBD); mast cells; reactive oxygen species (ROS); trans epithelial electrical resistance (TEER)

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