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ACS Chem Neurosci. 2019 Mar 20;10(3):1555-1564. doi: 10.1021/acschemneuro.8b00527. Epub 2018 Dec 7.

CuATSM Protects Against the In Vitro Cytotoxicity of Wild-Type-Like Copper-Zinc Superoxide Dismutase Mutants but not Mutants That Disrupt Metal Binding.

Author information

1
Illawarra Health and Medical Research Institute , Wollongong , NSW 2522 , Australia.
2
School of Biological Sciences, Centre of Medicine and Molecular Biosciences, Faculty of Science, Medicine and Health , University of Wollongong , Wollongong , NSW 2522 , Australia.
3
Department of Physics & Astronomy , The University of British Columbia , Vancouver , BC V6T 1Z1 , Canada.
4
Genome Sciences and Technology Program , The University of British Columbia , Vancouver , BC V6T 1Z2 , Canada.
5
Djavad Mowafaghian Centre for Brain Health , The University of British Columbia , Vancouver , BC V6T 2B5 , Canada.

Abstract

Mutations in the SOD1 gene are associated with some forms of familial amyotrophic lateral sclerosis (fALS). There are more than 150 different mutations in the SOD1 gene that have various effects on the copper-zinc superoxide dismutase (SOD1) enzyme structure, including the loss of metal binding and a decrease in dimer affinity. The copper-based therapeutic CuATSM has been proven to be effective at rescuing neuronal cells from SOD1 mutant toxicity and has also increased the life expectancy of mice expressing the human transgenes SOD1G93A and SOD1G37R. Furthermore, CuATSM is currently the subject of a phase I/II clinical trial in Australia as a treatment for ALS. To determine if CuATSM protects against a broad variety of SOD1 mutations, we used a well-established cell culture model of SOD1-fALS. NSC-34 cells expressing SOD1-EGFP constructs were treated with CuATSM and examined by time-lapse microscopy. Our results show a concentration-dependent protection of cells expressing mutant SOD1A4V over the experimental time period. We tested the efficacy of CuATSM on 10 SOD1-fALS mutants and found that while protection was observed in cells expressing pathogenic wild-type-like mutants, cells expressing a truncation mutant or metal binding region mutants were not. We also show that CuATSM rescue is associated with an increase in human SOD1 activity and a decrease in the level of SOD1 aggregation in vitro. In conclusion, CuATSM has shown to be a promising therapeutic for SOD1-associated ALS; however, our in vitro results suggest that the protection afforded varies depending on the SOD1 variant, including negligible protection to mutants with deficient copper binding.

KEYWORDS:

ALS; CuATSM; SOD1; copper; protein aggregation

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