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Poult Sci. 2018 Nov 20. doi: 10.3382/ps/pey515. [Epub ahead of print]

In vitro culture and characterization of duck primordial germ cells.

Author information

1
Institute of Biotechnology, National Taiwan University, Taipei 10672, Taiwan.
2
Univ Lyon, Université Lyon 1, Stem Cell and Brain Research Institute, U1208, USC1361, INSERM, INRA, Bron 69500, France.
3
Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan.
4
Ilan Branch, Livestock Research Institute, Council of Agriculture, Executive Yuan, Ilan 26846, Taiwan.
5
Livestock Research Institute, Council of Agriculture, Executive Yuan, Tainan 71246, Taiwan.
6
Department of Animal Science and Technology, National Taiwan University, Taipei 10672, Taiwan.

Abstract

This study aimed to isolate, culture, and characterize duck primordial germ cells (PGCs) and to compare these cells with chicken PGCs. We first cultured Muscovy duck (Cairina moschata) circulating PGCs and gonadal PGCs (gPGCs) in the modified serum-containing medium used to amplify chicken PGCs. gPGCs were found to proliferate better in serum-free chemically defined medium than in serum-containing medium. Thereafter, gPGCs were similarly isolated from 2 other duck breeds, the Pekin duck (Anas platyrhynchos) and the hybrid mule duck (C. moschata × A. platyrhynchos), and amplified for a limited period of time in the chemically defined culture condition, but sufficiently to be characterized and transplanted. Cultured gPGCs of all 3 duck breeds were characterized by Periodic acid-Schiff staining, immunocytochemical staining, and expression analysis of germline-specific and pluripotency genes. Cultured duck gPGCs colonized the gonads after being genetically labeled and injected into recipient embryos. Taken together, these results demonstrate that duck PGCs retain their germline characteristics after being isolated, expanded in vitro, and genetically modified. Further studies are required to establish the optimal conditions for long-term culture of duck PGCs, which may involve supplementing the culture medium with other growth factors or compounds.

PMID:
30462334
DOI:
10.3382/ps/pey515

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