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Elife. 2018 Nov 21;7. pii: e43022. doi: 10.7554/eLife.43022.

Random-sequence genetic oligomer pools display an innate potential for ligation and recombination.

Author information

1
MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
2
REGA Institute, Katholieke Universiteit Leuven, Leuven, Belgium.
#
Contributed equally

Abstract

Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes. Here, we describe an innate capacity for non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools. Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids). While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA. Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.

KEYWORDS:

RNA; biochemistry; chemical biology; ligation; non-enzymatic; none; nucleic acids; origin of life; recombination

PMID:
30461419
DOI:
10.7554/eLife.43022
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