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Nucleic Acids Res. 1988 Aug 11;16(15):7545-62.

Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein.

Author information

1
Department of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City 84132.

Abstract

We have sequenced the Escherichia coli grpE gene and shown that it encodes a 197-amino acid residue protein of 21,668-Mr. The predicted N-terminal amino acid sequence, as well as the overall amino acid composition agree well with that of the purified protein. From Northern analysis, we have shown that transcription of the grpE gene is under heat shock regulation, i.e., there is a rapid and transient increase in the rate of synthesis of grpE mRNA upon a shift-up in temperature. Forty-six bases upstream of the structural gene is a sequence closely related to the consensus heat shock promoter identified by Cowing et al. [Proc. Natl. Acad. Sci. U.S.A, 82, 2679-2683]. We have shown by S1 mapping and RNA sequencing that this is indeed the promoter for the grpE mRNA. It appears that all discernable transcription initiates only from this promoter, even under non-heat shock conditions.

PMID:
3045760
PMCID:
PMC338426
DOI:
10.1093/nar/16.15.7545
[Indexed for MEDLINE]
Free PMC Article

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