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Phytopathology. 2018 Nov 20. doi: 10.1094/PHYTO-07-18-0273-FI. [Epub ahead of print]

Distribution and genetic diversity of Xylella fastidiosa subsp. pauca associated with olive quick syndrome symptoms in Southeastern Brazil.

Author information

1
Centro de Citricultura Sylvio Moreira, Cordeiropolis, Sao Paulo, Brazil ; nagela.safady@hotmail.com.
2
ESALQ/Universidade de São Paulo, Entomologia e Acarologia , Av. Padua Dias, 11 , Piracicaba, SP, Brazil , 13418-900 ; jrslopes@usp.br.
3
Eidgenossische Technische Hochschule Zurich, 27219, Plant Pathology , Universitätstrasse 2 , Zurich, Switzerland , 8092 ; carolina.sardinha@usys.ethz.ch.
4
Centro de Citricultura Sylvio Moreira , Rod Anhanguera km 158 , Cordeiropolis, Brazil , 13490000 ; hdcoletta@ccsm.br.

Abstract

In Brazil, the host expansion of Xylella fastidiosa subsp. pauca was recently demonstrated with the report of diseased olive trees (Olea europaea), whose symptoms were associated with olive quick decline syndrome (OQDS) early describe in Southern Italy. We employed both PCR-based technique and the culture medium isolation to investigate the geographic dispersion of X. fastidiosa, as well as, the genetic signatures of 21 strains isolated from 11 olive orchards in both, São Paulo and Minas Gerais States, Brazil. Xylella fastidiosa subsp. pauca was detected in 83% of orchards examined in the region and was positively diagnosed in 43.7% of all samples with typical scorching symptoms. Of the 21 strains characterized by the fast-evolving microsatellite (SSR) markers, 20 different multilocus microsatellite genotypes (MLMGs) were observed with an overall allelic diversity of HNei = 0.38. Principal component analysis (PCoA) using the SSR markers clustered all strains, except for three, in one cluster evidencing a limited range of genetic diversity. Multilocus sequence typing (MLST) analysis shown the prevalence of a sequence type (ST16) in 75% of the samples. Three novel STs (84, 85, and 86) belonging to the X. fastidiosa subsp. pauca were detected. These results denote that genetically diverse strains of X. fastidiosa subsp. pauca are widely present in olive orchards in Southeastern Brazil, which is consistent with the long history of this bacterium in that region.

KEYWORDS:

Bacteriology; Ecology and epidemiology

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