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Med Vet Entomol. 2019 Mar;33(1):99-109. doi: 10.1111/mve.12343. Epub 2018 Nov 18.

Longitudinal monitoring of anti-saliva antibodies as markers of repellent efficacy against Phlebotomus perniciosus and Phlebotomus papatasi in dogs.

Author information

1
Department of Animal Health, Faculty of Veterinary Science, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, Spain.
2
Department of Parasitology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
3
Department of Medicine and Surgery, Faculty of Veterinary Science, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, Spain.
4
Molecular Biology Department, Institute of Parasitology and Biomedicine 'López Neyra', Consejo Superior de Investigaciones Científicas, Granada, Spain.
5
Bioorganic Research and Services, SA, Jerez de la Frontera, Spain.

Abstract

A 2-year longitudinal study of enzyme-linked immunosorbent assay (ELISA) antibodies against Phlebotomus perniciosus and Phlebotomus papatasi (Diptera: Psychodidae) sandfly saliva was performed in 32 Beagle dogs treated preventively with an imidacloprid-permethrin topical insecticide in an endemic area in Spain. Dogs were grouped into three sandfly exposure groups according to the time of inclusion in the study. Assays analysed immunoglobulin G (IgG) against salivary gland homogenates (SGH) of both species and recombinant P. papatasi rSP32 and P. perniciosus rSP03B proteins in serum. The dogs were participating in a Leishmania infantum (Kinetoplastida: Trypanosomatidae) vaccine trial and were experimentally infected with the parasite in the second year. No dog acquired natural L. infantum infections during the first year, but most developed anti-saliva antibodies, and median log-transformed optical densities (LODs) were seasonal, mimicking those of local sandflies. This indicates that the repellent efficacy of the insecticide used is below 100%. Multi-level modelling of LODs revealed variability among dogs, autocorrelation and differences according to the salivary antigen and the dog's age. However, dog seroprevalence, estimated using pre-exposure LODs as cut-offs, was relatively low. This, and the fact that dogs did not become naturally infected with L. infantum, would support the efficacy and usefulness of this imidacloprid-permethrin topical insecticide in canine leishmaniasis control.

KEYWORDS:

Antibodies; antigens; dogs; insecticides; saliva; sandfly

PMID:
30450832
DOI:
10.1111/mve.12343
[Indexed for MEDLINE]

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