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J Agric Food Chem. 2018 Dec 19;66(50):13260-13267. doi: 10.1021/acs.jafc.8b04659. Epub 2018 Dec 4.

Egg Yolk Immunoglobulin Supplementation Prevents Rat Liver from Aflatoxin B1-Induced Oxidative Damage and Genotoxicity.

Author information

1
Guangdong Provincial Key Laboratory of Food Quality and Safety/College of Food Science , South China Agricultural University , Guangzhou 510642 , China.
2
Key Laboratory of Environment and Female Reproductive Health/Public Health Laboratory Sciences and Toxicology, West China School of Public Health , Sichuan University , Chengdu , China.
3
Department of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment , Ministry of Health , Beijing 100021 China.
4
Institute of Toxicology, College of Preventive Medicine , Third Military Medical University , Chongqing , China.

Abstract

Egg yolk immunoglobulins (IgY), as nutraceutical supplement for therapeutic or prophylactic intervention, have been extensively studied. The effects of IgY on small molecular toxin-induced toxicity in animals are unclear. In the present study, the protection of highly purified and specific anti-AFB1 IgY against AFB1-induced genotoxicity and oxidative damage on the rat liver model were investigated. Our results revealed that AFB1 induced significant oxidative damage markers, as well as AFB1-induced protein expression in antioxidant, pro- and antiapoptosis processes in rat liver. These effects could be significantly inhibited by cogavage with anti-AFB1 IgY in a dose-dependent manner. However, anti-AFB1 IgY did not significantly induce hepatic CAT and SOD1. To explore mechanisms, metabolite experiments were established to evaluate the influence of anti-AFB1 IgY on the absorption of AFB1 in rats. Middle and high doses of anti-AFB1 IgY reduced hepatic AFB1-DNA adducts by 43.3% and 52.9%, AFB1- N7-guanine urinary adducts by 19.6% and 34.4%, and AFB1-albumin adducts by 10.5% and 21.1%, respectively. The feces of high dose anti-AFB1 IgY cogavaged rats contained approximately 2-fold higher AFB1 equivalents at 3-6 h after ingestion than AFB1 group feces, indicating IgY inhibited AFB1 uptake. These results had provided insight that anti-AFB1 IgY could prevent animal organs from damage caused by AFB1 and will be beneficial for the application of detoxification antibody as a supplement in food.

KEYWORDS:

Anti-AFB1 IgY; aflatoxin B1; genotoxicity; oxidative damage

PMID:
30449111
DOI:
10.1021/acs.jafc.8b04659

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