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Toxicol In Vitro. 2019 Mar;55:51-57. doi: 10.1016/j.tiv.2018.11.007. Epub 2018 Nov 15.

Poly(ADP-ribose) polymerase-1 promotes expression of miR-155 by the up-regulation of methyl-CpG binding domain protein 2 in TK6 cells exposed to hydroquinone.

Author information

1
Department of Urolgoy, The Affiliated Hospital of Guangdong Medical University, Guangdong Medical University, Zhanjiang 524001, PR China; Guangdong Key Laboratory of Urology, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510230, PR China.
2
Department of Environmental and Occupational Health, School of Public Health, Guangdong Medical University, Dongguan 523808, PR China; Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, PR China; School of Public Health, Guangdong Medical University, Dongguan 523808, PR China.
3
Foshan Institute of Occupational Disease Prevention and Control, Foshan, Guangdong 528000, PR China.
4
Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, PR China; School of Public Health, Guangdong Medical University, Dongguan 523808, PR China.
5
Huizhou Hospital for Occupational Disease Prevention and Treatment, Huizhou 516001, PR China.
6
Department of Histology and Embryology, Guangdong Medical University, Zhanjiang, PR China.
7
Department of Environmental and Occupational Health, School of Public Health, Guangdong Medical University, Dongguan 523808, PR China; Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, PR China. Electronic address: linhua-liu@163.com.

Abstract

Hydroquinone (HQ), one of the major metabolites of benzene, can induce aberrant gene expression. MiR-155, a tumor activator, participates in various biological processes, including DNA damage response. However, the molecular mechanism of aberrant miR-155 expression is still not completely elucidated. Here, we investigated the mechanism of abnormal expression of miR-155 induced by poly(ADP-ribose)polymerase-1 (PARP-1) expression in HQ-treated TK6 lymphoblastoid cells. We examined the expression of genes related to abnormal expression of miR-155 to explore the reason for this phenomenon. The results of the present study showed that miR-155 was significantly increased and reactive oxygen species (ROS) were decreased in cells treated with HQ for 72 h compared with PBS-treated cells. Meanwhile, E4F1, PARP-1 and PARP-1 related co-regulators (NF-κB, HDAC1, and HDAC2), acetylated histone H3 (H3Ac) were increased in a concentration-dependent manner. Experiments for treatment with 5-AzaC (DNMTs inhibitor), TSA (HDACs inhibitor), DOX (to activate PARP-1) or MG132 (proteasome inhibitor) revealed that the MBDs and PARP-1 was positively associated with miR-155 expression. Moreover, in cells treated with HQ in conjunction with PARP-1 knockdown, expression of miR-155, H3Ac and MBD2 protein were decreased, compared with negative control. In conclusion, PARP-1 activates expression of miR-155 via acetylation by regulating MBD2 in TK6 cells exposed to HQ.

KEYWORDS:

Histone modification; Hydroquinone; MBD2; PARP-1; miR-155

PMID:
30448557
DOI:
10.1016/j.tiv.2018.11.007
[Indexed for MEDLINE]

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