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EMBO Mol Med. 2018 Dec;10(12). pii: e8861. doi: 10.15252/emmm.201808861.

Rescuing ocular development in an anophthalmic pig by blastocyst complementation.

Zhang H1,2,3, Huang J1,2,3, Li Z4, Qin G1,2,3, Zhang N1, Hai T1,2,3, Hong Q1, Zheng Q1,2,3, Zhang Y1,2,3, Song R1,2,3, Yao J1,2,3, Cao C1,2,3, Zhao J5,2,3, Zhou Q5,2,3.

Author information

1
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
2
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China.
3
Institute of Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China.
4
College of Life Sciences Qufu Normal University, Qufu, China.
5
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China zhaojg@ioz.ac.cn qzhou@ioz.ac.cn.

Abstract

Porcine-derived xenogeneic sources for transplantation are a promising alternative strategy for providing organs for treatment of end-stage organ failure in human patients because of the shortage of human donor organs. The recently developed blastocyst or pluripotent stem cell (PSC) complementation strategy opens a new route for regenerating allogenic organs in miniature pigs. Since the eye is a complicated organ with highly specialized constituent tissues derived from different primordial cell lineages, the development of an intact eye from allogenic cells is a challenging task. Here, combining somatic cell nuclear transfer technology (SCNT) and an anophthalmic pig model (MITF L 247S/L247S), allogenic retinal pigmented epithelium cells (RPEs) were retrieved from an E60 chimeric fetus using blastocyst complementation. Furthermore, all structures were successfully regenerated in the intact eye from the injected donor blastomeres. These results clearly demonstrate that not only differentiated functional somatic cells but also a disabled organ with highly specialized constituent tissues can be generated from exogenous blastomeres when delivered to pig embryos with an empty organ niche. This system may also provide novel insights into ocular organogenesis.

KEYWORDS:

anophthalmic pig; chimera; organ regeneration; somatic cell nuclear transfer technology

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