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J Mol Cell Cardiol. 2019 Jan;126:13-22. doi: 10.1016/j.yjmcc.2018.11.004. Epub 2018 Nov 13.

Identification and regulation of the long non-coding RNA Heat2 in heart failure.

Author information

1
Institute for Cardiovascular Regeneration, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany; Department of Cardiology, Internal Medicine III, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany; Clinic and Polyclinic for Cardiology, University Hospital Leipzig, Germany. Electronic address: Jes-Niels.Boeckel@medizin.uni-leipzig.de.
2
Institute for Cardiovascular Regeneration, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany.
3
Institute for Cardiovascular Regeneration, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany; German Centre for Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt, Germany.
4
Department of Cardiology, Internal Medicine III, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany.
5
Laboratory for Novel sequencing technology, Functional and Medical Genomics, Berlin Institute for Medical Systems Biology, Max-Delbrück-Centrum for Molecular Medicine, Berlin, Germany; Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China.
6
Clinic and Polyclinic for Cardiology, University Hospital Leipzig, Germany.
7
Clinic and Polyclinic for Cardiology, University Hospital Leipzig, Germany; German Centre for Cardiovascular Research (DZHK), Partner site Heidelberg, Heidelberg, Germany.
8
Medical Oncology, NCT Heidelberg, Heidelberg, Germany; Hamamatsu Tissue Imaging and Analysis Center, Bioquant, University Heidelberg, Heidelberg, Germany.
9
German Centre for Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt, Germany; Heart center, Cardiology department, University Göttingen, Germany.
10
Division for Pediatric Stem-Cell Transplantation and Immunology, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany.
11
Department of Cardiology, Internal Medicine III, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany; German Centre for Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt, Germany.
12
Department of Cardiology, Internal Medicine III, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany; German Centre for Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt, Germany; Kerckhoff Heart and Thorax Center, Department of Cardiology, Bad Nauheim, Germany.

Abstract

AIMS:

Circulating immune cells have a significant impact on progression and outcome of heart failure. Long non-coding RNAs (lncRNAs) comprise novel epigenetic regulators which control cardiovascular diseases and inflammatory disorders. We aimed to identify lncRNAs regulated in circulating immune cells of the blood of heart failure patients.

METHODS AND RESULTS:

Next-generation sequencing revealed 110 potentially non-coding RNA transcripts differentially expressed in peripheral blood mononuclear cells of heart failure patients with reduced ejection fraction. The up-regulated lncRNA Heat2 was further functionally characterized. Heat2 expression was detected in whole blood, PBMNCs, eosinophil and basophil granulocytes. Heat2 regulates cell division, invasion, transmigration and immune cell adhesion on endothelial cells.

CONCLUSION:

Heat2 is an immune cell enriched lncRNA that is elevated in the blood of heart failure patients and controls cellular functions.

KEYWORDS:

Eosinophils; Heart failure; Immune cells; lncRNAs

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