Long noncoding RNA MAGI2-AS3 regulates CCDC19 expression by sponging miR-15b-5p and suppresses bladder cancer progression

Feng Wang; Yanwen Zu; Shibin Zhu; Yu Yang; Weiping Huang; Hui Xie; Gonghui Li

doi:10.1016/j.bbrc.2018.11.013

Long noncoding RNA MAGI2-AS3 regulates CCDC19 expression by sponging miR-15b-5p and suppresses bladder cancer progression

Biochem Biophys Res Commun. 2018 Dec 9;507(1-4):231-235. doi: 10.1016/j.bbrc.2018.11.013. Epub 2018 Nov 12.

Authors

Feng Wang¹, Yanwen Zu², Shibin Zhu³, Yu Yang⁴, Weiping Huang⁴, Hui Xie⁴, Gonghui Li⁵

Affiliations

¹ Department of Urology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China; Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
² Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, 325035, China.
³ Department of Urology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China.
⁴ Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
⁵ Department of Urology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China. Electronic address: gonghuilii11@163.com.

PMID: 30442369
DOI: 10.1016/j.bbrc.2018.11.013

Abstract

Bladder cancer (BCa) belongs to a popular urological malignancy and leads to large numbers of deaths worldwide. Recently, emerging evidences indicate that long noncoding RNAs (lncRNAs) are closely related with BC occurrence and progression. However, the function of lncRNA MAGI2-AS3 remains poorly understood in BC. In this present study, we screened out a novel lncRNA MAGI2-AS3 whose expression was downregulated in BCa tissues. We showed that MAGI2-AS3 downregulation in BCa patients indicated a poor prognosis. Functionally, we showed that MAGI2-AS3 overexpression inhibits proliferation, migration and invasion of BCa cells. Moreover, ectopic expression of MAGI2-AS3 suppresses BCa growth in vivo. Bioinformatics analysis revealed that MAGI2-AS3 could serve as a competing endogenous RNA (ceRNA) for miR-15b-5p. In the meantime, miR-15b-5p directly targeted CCDC19, a tumor suppressor in BCa. Rescue assays demonstrated that knockdown of CCDC19 restored the proliferation, migration and invasion of BCa cells suppressed by MAGI2-AS3 overexpression. In conclusion, this study identified a novel mechanism that MAGI2-AS3/miR-15b-5p/CCDC19 signaling pathway regulates BCa progression.

Keywords: Bladder cancer; CCDC19; MAGI2-AS3; Progression; miR-15b-5p.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cell Line, Tumor
Cell Proliferation / genetics
Cytoskeletal Proteins
Disease Progression*
Female
Gene Expression Regulation, Neoplastic*
Gene Knockdown Techniques
Humans
Mice, Inbred BALB C
Mice, Nude
MicroRNAs / genetics
MicroRNAs / metabolism*
Proteins / genetics*
Proteins / metabolism
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism*
Urinary Bladder Neoplasms / genetics*
Urinary Bladder Neoplasms / pathology*

Substances

CFAP45 protein, human
Cytoskeletal Proteins
MIRN15 microRNA, human
MicroRNAs
Proteins
RNA, Long Noncoding