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Int J Biol Macromol. 2019 Feb 15;123:531-538. doi: 10.1016/j.ijbiomac.2018.11.087. Epub 2018 Nov 13.

Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L.

Author information

1
College of Science, Sichuan Agriculture University, Ya'an 625014, China.
2
Guizhou Business and Economics Vocational College of Technology, Duyun 558022, China.
3
Qiannan Normal University for Nationalities, Duyun 558000, China.
4
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
5
Agricultural Information and Rural Economy Institute of Sichuan Academy of Agricultural Science, Chengdu 610066, China.
6
College of Science, Sichuan Agriculture University, Ya'an 625014, China. Electronic address: zhangli@sicau.edu.cn.

Abstract

Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication. A central composition design was used to optimize extraction parameters by maximizing the polysaccharide extraction yield. The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification. Results indicated that the three fractions had a molecular weight of 218, 178, and 60 kDa, respectively, and were composed of mannose, glucose, fructose, and galactose. ALP60-1 exhibited strong scavenging activities on 1,1‑diphenyl‑2‑picryhydrazyl, hydroxyl, and superoxide radicals. These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant. However, further structure elucidation and in vivo experiments are required.

KEYWORDS:

Antioxidant activities; Arctium lappa L.; Polysaccharides; Ultrasonic-assisted extraction

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