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Nature. 2018 Nov;563(7732):501-507. doi: 10.1038/s41586-018-0692-z. Epub 2018 Nov 14.

Improved reference genome of Aedes aegypti informs arbovirus vector control.

Author information

1
Laboratory of Neurogenetics and Behavior, The Rockefeller University, New York, NY, USA. bnmtthws@gmail.com.
2
Howard Hughes Medical Institute, New York, NY, USA. bnmtthws@gmail.com.
3
Kavli Neural Systems Institute, New York, NY, USA. bnmtthws@gmail.com.
4
The Center for Genome Architecture, Baylor College of Medicine, Houston, TX, USA.
5
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
6
Department of Computer Science, Rice University, Houston, TX, USA.
7
Center for Theoretical and Biological Physics, Rice University, Houston, TX, USA.
8
Pacific Biosciences, Menlo Park, CA, USA.
9
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.
10
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
11
Verily Life Sciences, South San Francisco, CA, USA.
12
Mortimer B. Zuckerman Mind Brain Behavior Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
13
Laboratory of Neurogenetics and Behavior, The Rockefeller University, New York, NY, USA.
14
Kavli Neural Systems Institute, New York, NY, USA.
15
Broad Institute of MIT and Harvard, Cambridge, MA, USA.
16
Department of Immunology and Infectious Disease, Harvard T. H. Chan School of Public Health, Boston, MA, USA.
17
Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA.
18
Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA.
19
Vector Biology Department, Liverpool School of Tropical Medicine, Liverpool, UK.
20
Liverpool John Moores University, Liverpool, UK.
21
Department of Pathogen Biology, School of Public Health, Southern Medical University, Guangzhou, China.
22
Department of Biochemistry, Virginia Tech, Blacksburg, VA, USA.
23
Fralin Life Science Institute, Virginia Tech, Blacksburg, VA, USA.
24
Department of Entomology, Purdue University, West Lafayette, IN, USA.
25
Purdue Institute for Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, IN, USA.
26
Centre for Respiratory Biology, UCL Respiratory, University College London, London, UK.
27
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA.
28
Bionano Genomics, San Diego, CA, USA.
29
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA.
30
Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, USA.
31
Vector Biology and Control Section, Department of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand.
32
Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
33
Insect-Virus Interactions Group, Department of Genomes and Genetics, Institut Pasteur, Paris, France.
34
Unité de Parasitologie et Entomologie, Département des Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, Marseille, France.
35
Centre National de la Recherche Scientifique, Unité Mixte de Recherche 2000, Paris, France.
36
Aix Marseille Université, IRD, AP-HM, SSA, UMR Vecteurs - Infections Tropicales et Méditerranéennes (VITROME), IHU - Méditerranée Infection, Marseille, France.
37
The Connecticut Agricultural Experiment Station, New Haven, CT, USA.
38
Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Oxford, UK.
39
Department of Entomology, University of California Riverside, Riverside, CA, USA.
40
Department of Bioengineering, Rice University, Houston, TX, USA.
41
Department of Pediatrics, Texas Children's Hospital, Houston, TX, USA.
42
Department of Entomology, Center for Disease Vector Research and Institute for Integrative Genome Biology, University of California, Riverside, CA, USA.
43
Department of Entomology, Virginia Tech, Blacksburg, VA, USA.
44
Institute of Integrative Biology, University of Liverpool, Liverpool, UK.
45
Division of Biological Sciences, University of California, San Diego, La Jolla, CA, USA.
46
Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA.
47
Department of Entomology, Texas A&M University, College Station, TX, USA.
48
Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State University, Tomsk, Russia.
49
Laboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, NY, USA.
50
Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
51
Howard Hughes Medical Institute, New York, NY, USA.

Abstract

Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector.

PMID:
30429615
DOI:
10.1038/s41586-018-0692-z

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