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Mol Biol Cell. 2019 Jan 15;30(2):256-267. doi: 10.1091/mbc.E18-05-0279. Epub 2018 Nov 14.

The J-domain cochaperone Rsp1 interacts with Mto1 to organize noncentrosomal microtubule assembly.

Shen J1,2,3, Li T1,2,3, Niu X1,2,3, Liu W1,2,3, Zheng S1,2,3, Wang J1,2,3, Wang F4, Cao X4, Yao X1,2,3, Zheng F1,2,3, Fu C1,2,3.

Author information

1
Division of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
2
Chinese Academy of Sciences Center for Excellence in Molecular Cell Sciences, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
3
Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, University of Science and Technology of China, Hefei 230027, China.
4
School of Life Sciences, Anhui Medical University, Hefei, Anhui 230027, China.

Abstract

Microtubule biogenesis initiates at various intracellular sites, including the centrosome, the Golgi apparatus, the nuclear envelope, and preexisting microtubules. Similarly, in the fission yeast Schizosaccharomyces pombe, interphase microtubules are nucleated at the spindle pole body (SPB), the nuclear envelope, and preexisting microtubules, depending on Mto1 activity. Despite the essential role of Mto1 in promoting microtubule nucleation, how distribution of Mto1 in different sites is regulated has remained elusive. Here, we show that the J-domain cochaperone Rsp1 interacts with Mto1 and specifies the localization of Mto1 to non-SPB nucleation sites. The absence of Rsp1 abolishes the localization of Mto1 to non-SPB nucleation sites, with concomitant enrichment of Mto1 to the SPB and the nuclear envelope. In contrast, Rsp1 overexpression impairs the localization of Mto1 to all microtubule organization sites. These findings delineate a previously uncharacterized mechanism in which Rsp1-Mto1 interaction orchestrates non-SPB microtubule formation.

PMID:
30427751
PMCID:
PMC6589567
DOI:
10.1091/mbc.E18-05-0279
[Indexed for MEDLINE]
Free PMC Article

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