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Mol Biol Rep. 2018 Nov 13. doi: 10.1007/s11033-018-4448-5. [Epub ahead of print]

Reciprocal regulation of pro-inflammatory Annexin A2 and anti-inflammatory Annexin A1 in the pathogenesis of rheumatoid arthritis.

Author information

1
Arthritis Superspeciality Center, Hubli, Karnataka, India.
2
Department of Medicine, SDM College of Medical Sciences and Hospital, Dharwad, India.
3
Central Research Lab, SDM College of Medical Sciences and Hospital, Sattur, Dharwad, Karnataka, 580 009, India. shettybp@hotmail.com.
4
Department of Biochemistry, SDM College of Medical Sciences and Hospital, Sattur, Dharwad, Karnataka, 580 009, India. shettybp@hotmail.com.
5
Central Research Lab, SDM College of Medical Sciences and Hospital, Sattur, Dharwad, Karnataka, 580 009, India.
6
Department of Biochemistry, SDM College of Medical Sciences and Hospital, Sattur, Dharwad, Karnataka, 580 009, India.
7
Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX, USA.
8
Department of Pathology, SDM College of Medical Sciences and Hospital, Dharwad, Karnataka, India.

Abstract

Annexin A2 has been implicated in several immune modulated diseases including Rheumatoid arthritis (RA) pannus formation. The most relied treatment option for RA pathogenesis is glucocorticoids. Glucocorticoids regulate the synthesis, phosphorylation and cellular deposition of Annexin A1. This annexin mediates the anti-inflammatory actions of glucocorticoids. These two first characterized members of annexin superfamily proteins acts reciprocally, one as an anti-inflammatory and the other proinflammatory in nature. The possibility of these molecules as soluble biomarkers and as an upstream regulator of major cytokine devastation at RA microenvironment has not been previously explored. Current study elucidates the reciprocal regulation of these two annexins in RA pathogenesis. These Annexin A2/A1 and downstream cytokines in RA serum were analysed by ELISA. Western blot, Immunocytochemistry, immunoprecipitation and Immunohistochemistry were adapted to analyse these molecules in tissue and synovial fibroblasts and also in different experimental conditions. Significant increase in the level of Annexin A2 was noticed in naïve RA patients compared to controls (14.582 ± 1.766 ng/ml vs. 7.37 ± 1.450 ng/ml; p ≤ 0.001). In remission cases significant low levels was detected. On the contrary, significant decrease in the level of Annexin A1 was noticed in naïve RA patients compared to healthy controls (12.322 ± 2.91 vs. 16.998 ± 4.298 ng/ml; p ≤ 0.001), wherein remission cases serum Annexin A1 was significantly high. The knockdown of proinflammatory Annexin A2 by siRNA/antibody treatment could mimic the glucocorticoid treatment as which induced cellular Annexin A1 and membrane translocation resulting in the terminal action. Current data elucidating the regulatory interplay between Annexin A2 and Annexin A1 in RA pathogenesis.

KEYWORDS:

Annexin A1; Annexin A2; Inflammatory cytokines; Proinflammatory mediators; Rheumatoid arthritis

PMID:
30426384
DOI:
10.1007/s11033-018-4448-5

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