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Eur J Clin Microbiol Infect Dis. 2018 Nov 13. doi: 10.1007/s10096-018-3415-3. [Epub ahead of print]

Evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying Burkholderia pseudomallei and Burkholderia thailandensis isolates.

Author information

1
Department of Laboratory Medicine, Institute of Surgery Research, Daping Hospital, Army Medical University, Chongqing, 400042, People's Republic of China.
2
Department of Respiratory and Critical Care Medicine, The first affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
3
Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Army Medical University, Chongqing, 400038, People's Republic of China.
4
Department of Laboratory Medicine, Institute of Surgery Research, Daping Hospital, Army Medical University, Chongqing, 400042, People's Republic of China. luweiping19710416@163.com.

Abstract

Since Burkholderia thailandensis is included in the reference spectra of the VITEK MS libraries rather than Burkholderia pseudomallei, B. pseudomallei cannot be correctly identified in the current version of VITEK MS. This study was undertaken to evaluate the utility of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with the VITEK MS plus system in the detection of B. pseudomallei and B. thailandensis isolates. For each species, we increased the reference spectra, and then, a SuperSpectrum was created based on the selection of 39 specific masses. In a second step, we validated the SuperSpectra with 106 isolates identified by 16S rRNA gene sequencing. The results showed that there was 100% agreement between the validation strains analyzed by MALDI-TOF MS and those evaluated using 16S rRNA gene sequencing analysis methods. Therefore, MALDI-TOF MS is a promising, rapid, and economical method to monitor the outbreaks and spread of B. pseudomallei and B. thailandensis isolates.

KEYWORDS:

16S rRNA gene sequencing analysis; Burkholderia pseudomallei; Burkholderia thailandensis; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; SuperSpectra

PMID:
30426332
DOI:
10.1007/s10096-018-3415-3

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