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J Exp Clin Cancer Res. 2018 Nov 13;37(1):275. doi: 10.1186/s13046-018-0936-7.

CircRNA circRNA_102171 promotes papillary thyroid cancer progression through modulating CTNNBIP1-dependent activation of β-catenin pathway.

Author information

1
Department of Head and Neck Surgery, The Third Affiliated Hospital of Harbin Medical University, No.150 Haping Road, Nangang District, Harbin, 150081, China.
2
Department of Pediatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, 150070, China.
3
Department of Cell Biology, Harbin Medical University, No.157 Baojian Road, Nangang District, Harbin, 150081, China. jinxujx67@163.com.
4
Department of Head and Neck Surgery, The Third Affiliated Hospital of Harbin Medical University, No.150 Haping Road, Nangang District, Harbin, 150081, China. Zhang_Jwu_nic@126.com.

Abstract

BACKGROUND:

As a type of recently discovered noncoding RNA, circular RNAs (circRNAs) exert pivot biological functions in diverse cancers. However, the role of circRNA_102171 in papillary thyroid cancer (PTC) has not been investigated. Our study was focused on the functional investigation toward circRNA_102171 in PTC progression. And we also aimed to reveal its potential molecular mechanism.

METHODS:

The expression pattern of circRNA_102171 was determined using quantitative polymerase chain reaction (qPCR) in PTC samples and cell lines. Cell proliferation was examined utilizing CCK8, colony formation and EdU incorporation assays. Apoptosis was analyzed by Annexin V/PI staining and FACS detection. Cell migration and invasion was measured using Transwell assay. Tumor growth in vivo was determined through a xenograft assay. RNA-pulldown, RNA-IP (RIP) and RNA-EMSA were used to analyze the interaction between circRNA_102171 and CTNNBIP1.

RESULTS:

CircRNA_102171 expression was upregulated in tumor tissues and cell lines. CircRNA_102171 silencing suppressed PTC cell proliferation, migration and invasion while promoting apoptosis. CircRNA_102171 knockdown inhibited PTC growth in vivo. CircRNA_102171 interacted with CTNNBIP1 to block its interaction with the β-catenin/TCF3/TCF4/LEF1 complex, leading to activation of Wnt/β-catenin pathway.

CONCLUSIONS:

CircRNA_102171 overexpression promotes PTC progression through activating Wnt/β-catenin pathway in a CTNNBIP1-dependent way.

KEYWORDS:

CTNNBIP1; Circular RNA; Papillary thyroid cancer; Wnt/β-catenin; circRNA_102171

PMID:
30424816
PMCID:
PMC6234664
DOI:
10.1186/s13046-018-0936-7
[Indexed for MEDLINE]
Free PMC Article

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