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Micromachines (Basel). 2018 Apr 1;9(4). pii: E157. doi: 10.3390/mi9040157.

A Microfluidic Chip with Double-Slit Arrays for Enhanced Capture of Single Cells.

Author information

1
School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, China. 1700461@stu.neu.edu.cn.
2
School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, China. 1710120@stu.neu.edu.cn.
3
School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, China. wdysend@gmail.com.
4
School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, China. 1600460@stu.neu.edu.cn.
5
School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, China. 1500445@stu.neu.edu.cn.
6
School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, China. gydu@mail.neu.edu.cn.
7
School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, China. dchba@mail.neu.edu.cn.
8
Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA. qlin@columbia.edu.
9
Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. meiqi_tj@hust.edu.cn.
10
Shenyang Aeroengine Research Institute, Aviation Industry Corporation of China, Shenyang 110015, China. 1700459@stu.neu.edu.cn.
11
China National Heavy Duty Truck Group Co., Ltd., Jinan 250101, China. suda@jszx.com.
12
School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, China. meiqi_tj@hust.edu.cn.

Abstract

The application of microfluidic technology to manipulate cells or biological particles is becoming one of the rapidly growing areas, and various microarray trapping devices have recently been designed for high throughput single-cell analysis and manipulation. In this paper, we design a double-slit microfluidic chip for hydrodynamic cell trapping at the single-cell level, which maintains a high capture ability. The geometric effects on flow behaviour are investigated in detail for optimizing chip architecture, including the flow velocity, the fluid pressure, and the equivalent stress of cells. Based on the geometrical parameters optimized, the double-slit chip enhances the capture of HeLa cells and the drug experiment verifies the feasibility of the drug delivery.

KEYWORDS:

cell capture; drug delivery; hydrodynamic cell trapping; microfluidic chip; single cell

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