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Materials (Basel). 2018 Nov 9;11(11). pii: E2234. doi: 10.3390/ma11112234.

Bioactive Sphene-Based Ceramic Coatings on cpTi Substrates for Dental Implants: An In Vitro Study.

Author information

1
Department of Industrial Engineering, University of Padova, Via F. Marzolo 9, 35131 Padova, Italy. hamada.elsayed@unipd.it.
2
Ceramics Department, National Research Centre, El-Bohous Street, 12622 Cairo, Egypt. hamada.elsayed@unipd.it.
3
Department of Management and Engineering, University of Padova, Stradella San Nicola 3, 36100 Vicenza, Italy. giulia-bru@libero.it.
4
Department of Neurosciences, Section of Dentistry University of Padova, Via Giustiniani 2, 35128 Padova, Italy. giulia-bru@libero.it.
5
Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy. chiara.gardin@unipd.it.
6
Maria Cecilia Hospital, GVM Care & Research, 48033 Ravenna, Italy. chiara.gardin@unipd.it.
7
Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy. letizia.ferroni@unipd.it.
8
Maria Cecilia Hospital, GVM Care & Research, 48033 Ravenna, Italy. letizia.ferroni@unipd.it.
9
Department of Chemical Sciences, University of Padova, Via F. Marzolo 1, 35131 Padova, Italy. denis.badocco@unipd.it.
10
Department of Chemical Sciences, University of Padova, Via F. Marzolo 1, 35131 Padova, Italy. paolo.pastore@unipd.it.
11
Department of Neurosciences, Section of Dentistry University of Padova, Via Giustiniani 2, 35128 Padova, Italy. stefano.sivolella@unipd.it.
12
Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy. barbara.zavan@unipd.it.
13
Maria Cecilia Hospital, GVM Care & Research, 48033 Ravenna, Italy. barbara.zavan@unipd.it.
14
Department of Management and Engineering, University of Padova, Stradella San Nicola 3, 36100 Vicenza, Italy. lisa.biasetto@unipd.it.

Abstract

Titanium implant surface modifications have been widely investigated to favor the process of osseointegration. The present work aimed to evaluate the effect of sphene (CaTiSiOâ‚…) biocoating, on titanium substrates, on the in vitro osteogenic differentiation of Human Adipose-Derived Stem Cells (hADSCs). Sphene bioceramic coatings were prepared using preceramic polymers and nano-sized active fillers and deposited by spray coating. Scanning Electron Microscopy (SEM) analysis, surface roughness measurements and X-ray diffraction analysis were performed. The chemical stability of the coatings in Tris-HCl solution was investigated. In vitro studies were performed by means of proliferation test of hADSCs seeded on coated and uncoated samples after 21 days. Methyl Thiazolyl-Tetrazolium (MTT) test and immunofluorescent staining with phalloidin confirmed the in vitro biocompatibility of both substrates. In vitro osteogenic differentiation of the cells was evaluated using Alizarin Red S staining and quantification assay and real-time PCR (Polymerase Chain Reaction). When hADSCs were cultured in the presence of Osteogenic Differentiation Medium, a significantly higher accumulation of calcium deposits onto the sphene-coated surfaces than on uncoated controls was detected. Osteogenic differentiation on both samples was confirmed by PCR. The proposed coating seems to be promising for dental and orthopedic implants, in terms of composition and deposition technology.

KEYWORDS:

bioactive ceramic coatings; biocompatibility; implant; osseointegration; sphene; titanium

PMID:
30424012
DOI:
10.3390/ma11112234
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